Microplate Reader Enzyme Reader Elisa

• Classification: Biological Diagnostics
• Type: Automatic Nucleic Acid Extraction Instrument
• Certification: CE, FDA, MSDS, ISO13485
• Group: Middle-aged and Old
• Transport Package: Carton
• Specification: 58*33*36cm
• Trademark: Runmei
• Origin: China
• Production Capacity: 1000sets/Month

Product Description

IgG/IgM
Antibody IgG/IgM and antigen detection test


1.Open the ELISA Kit, and take out all the contents, and leave the contents at room temperature for 30 minutes before use.

2.Check the Concentrated Washing Buffer, if there is precipitation in it, dissolve the precipitate at 37ºC before use. Diluted the Concentrated Washing Buffer 20 times with purified water to make the Working Washing buffer .

3.Take the required amount of Enzyme-Labeled Slats, seal unused slats as soon as possible, and store them at 2~8ºC.

4.Set one blank, one positive control well, and two negative control wells for each test. No liquid is added to the blank well; add 100μL the negative and positive controls to the negative and positive control wells, respectively; add the 100μL Sample Diluent to each remaining well respectively, then add 10 μL of the sample to each remaining well, mix well and then covered with Microplate Sealers, and incubate at 37°C for 30 minutes.

5.Remove the liquid in the wells, then add 300 μL of Working Washing Buffer to each well, Discard the buffer after 5-10 seconds, repeat washing for 5 times and then pat it on the table to make it dry.

Add 100μL of Enzyme Conjugate to each well. Covered with Microplate Sealers and incubate at 37°C for 20 minutes.

Remove the liquid in the wells, then add 300 μL of Working Washing Buffer to each well, Discard the buffer after 5-10 seconds, repeat washing for 5 times and then pat it on the table to make it dry.

Add 50 μL of Chromogenic Reagent A and Chromogenic Reagent B to each well and mix thoroughly, Covered with Microplate Sealers , and incubate at 37°C in dark for 10 minutes.


Add 50 μL of Stop Solution to each well and mix thoroughly.




Read data

1 Take out the Palmar Microplate Reader, press the "Power Button" for more than 3 second to start the machine, and then click the "Login" to login in the system.

2 Click "Test" on the screen and select "OD", select 450nm for main wave length and none for sub wave length to test.

3 Take out the card slot, and insert the Enzyme-Labeled Slats into the card slot, and put it into the machine.

4 Click the "Test" on the screen to start reading the OD value.

5 Click the "Print" on the screen to obtain the OD value data.

6 Remove the Enzyme-Labeled Slats and throw them away.

7 Click the "Settings" on the screen and then click the "Shut down" to shut down the machine.