| Customization: | Available |
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| Classification: | Biological Diagnostics |
| Type: | Runmei Gene |
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Creatine Kinase Assay Kit (Continuous Monitoring Method)
Creatine Kinase Activity Assay Kit - Data Sheet |
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| Kit Includes | Assay Buffer: 12 mL Substrate Solution: 1.0 mL Enzyme Mix: 120 mL Calibrator: 150 mL |
| Kit Requires | Pipeting (multi-channel) devices. Clear-bottom 96-well plates and plate reader. |
| Method of Detection | OD340nm |
| Detection Limit | 5 U/L |
| Samples | Serum, plasma etc |
| Species | All |
| Protocol Length | 5 min |
| Size | 100 tests |
| Storage | Store all reagents at -20°C |
| Shelf Life | 6 months |
CREATINE KINASE (CK), also known as creatine phosphokinase (CPK), is an enzyme (EC 2.7.3.2) expressed predominantly in skeletal muscle, smooth muscle and the brain. The CK enzyme consists of two subunits, which can be either B (brain type) or M (muscle type), and hence three different isoenzymes: CK-MM, CK-BB and CK-MB. CK catalyzes the conversion of creatine to phosphocreatine, consuming adenosine triphosphate (ATP) and generating adenosine diphosphate (ADP) and the reverse reaction. CK is often determined routinely in emergency patients with chest pain and acute renal failure. Elevation of CK is an indication of damage to muscle and has been associated with injury, rhabdomyolysis, myocardial infarction, myositis, myocarditis, malignant hyperthermia and neuroleptic malignant syndrome, etc. Lower levels can be an indication of alcoholic liver disease and rheumatoid arthritis. Simple, direct and automation-ready procedures for measuring CK activity are very desirable.
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