Monkeypox Virus Detection Kit (PCR-Fluorescent Probe)
Composition:
MPXV PCR Reaction Solution,MPXV Enzyme Mixture,MPXV Negative Control,MPXV Positive Control.
Applicable instrument:
This kit is suitable for ABI series, Bio-Rad series, Agilent Stratagene MX series, Roche LightCycler R480, Cepheid
SmartCycler, Rotor-Gene series and other multi-channel real-time quantitative PCR instruments.
Intended Use:
This kit is used for the detection of Monkeypoxvirus Nucleic Acid and assisted diagnosis and epidemiological
surveillance of Monkeypox.
Package Specifications:
50 tests/kit & 200 tests/box.
Storage temperature:
-20 ± 5ºC.
Period of validity:
12 Months.
Product Features:
1.Accuracy-The limit of detection is 100 copies/ml and the specificity is 100%.
2.Flexible- Skin lesion material, including swabs of lesion surface and/or exudate, roofs from more than one lesion, or
lesion crusts. Swab the lesion vigorously, to ensure adequate viral DNA is collected.
3.Simple-Simple operation,easy to.
4.Use Fast-Results within 40 min.
Interpretation of results:
1. If the test sample detects a typical S-type amplification curve in the FAM channels and the Ct value is ≤35, and there
is a typical S-type amplification curve in the Internal Control channel (Cy5), the sample can be judged to be MPXV
positive.
2. If the test sample has no amplification curve or Ct value> 38 in the FAM channels, and there is a typical S-type ampli
fication curve in the Internal Control channel (Cy5), the sample can be judged to be MPXV negative.
3. If the test sample yields a Ct value range is 35-38, and there is a typical S-type amplification curve in the Internal
Control channel (Cy5), the results need to retest. If the results repeated are consistent, and has a typical S-type amplifi
cation curve, the sample can be judged positive, otherwise, the sample can be judged as negative.
4. If no typical S-type amplification curve (No Ct value) or Ct value> 38 is detected in the FAM and Cy5 channels of the
test sample, it means that there is a problem with the quality of the sample or a problem with the operation. If the result
is invalid, you should find and eliminate the cause, collect the sample again, and repeat the test (if the test result is still
invalid, please contact the company)
Audited Supplier 
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