| Customization: | Available |
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| Type: | IVD Reagent |
| Package: | Not Customized |
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| Product parameter | |||||||||||||||
| composition:Enzyme Conjugate,Calibration Solution (S0-S5),20X Wash Buffer Concentrate,Substrate Reagent A,Substrate Reagent B,Stop Solution,Capture plate,Sealer,Instructions,Plastic Bag | |||||||||||||||
| Packing specification | |||||||||||||||
| 96 tests / kit | |||||||||||||||
| Storage temperature: 2-8ºC | |||||||||||||||
| Period of validity:Tentatively 12 months | |||||||||||||||
| Intened use | |||||||||||||||
| Neutralizing Antibody Detection Kit(ELISA ) is an indirect enzyme-linked immunoassay (ELISA) for quantitative determination of Neutralizing Antibody in plasma or serum. This kit is used for assisted diagnosis. | |||||||||||||||
| Priciple of the assay | |||||||||||||||
| Neutralizing Antibody Detection Kit(ELISA ) is a for rapid Nab (neutralizing antibody) screening, which can mimic the virus neutralization process. The kit contains two key components: the Horseradish peroxidase (HRP) conjugated recombinant fragment (HRP-RBD) and the human ACE2 receptor protein (hACE2). The protein-protein interaction between HRP-RBD and hACE2 can be blocked by neutralizing antibodies against RBD. First, the samples and controls are pre-incubated with the HRP-RBD to allow the binding of the circulating neutralization antibodies to HRP-RBD. The mixture is then added to the capture plate which is pre-coated with the hACE2 protein. The unbound HRP-RBD as well as any HRP-RBD bound to non-neutralizing antibody will be captured on the plate, while the circulating neutralization antibodies_HRP-RBD complexes remain in the supernatant and get removed during washing. After washing steps, TMB solution is added. By adding Stop Solution, the reaction is quenched. This final solution can be read at 450 nm in a microtiter plate reader. The absorbance of the sample is inversely dependent on the titer of the anti neutralizing antibodies. |
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